whole genome sequencing  (ATCC)

Journal: Scientific Reports

Article Title: A new Epseptimavirus bacteriophage vB_SalS-SIY1lw as a potential antimicrobial alternative to multidrug-resistant Salmonella Infantis

doi: 10.1038/s41598-025-31311-8

Figure Lengend Snippet: Whole genome sequence-based phylogenetic tree constructed using VICTOR (formula d0) on SIY1lw (with a red star) and the reference phages belonging to the Epseptimavirus and Tequintavirus genera, both under the Demerecviridae family. Escherichia phage vB_EcoS-UDF157lw used as an outgroup. The family, genus, and species are classified into different clusters with colors based on VICTOR analysis. Genomic GC content and sequence length are represented in the hue of color and horizontal black lines, respectively, on the right side of the tree.

Article Snippet: DNA library preparation was prepared based on the previously published protocol and subsequently loaded on a MiSeq Reagent Kit v2 (500-cycle) for whole-genome sequencing using the MiSeq platform (Illumina, San Deigo, CA, USA).

Techniques: Sequencing, Construct

Journal: Scientific Reports

Article Title: A new Epseptimavirus bacteriophage vB_SalS-SIY1lw as a potential antimicrobial alternative to multidrug-resistant Salmonella Infantis

doi: 10.1038/s41598-025-31311-8

Figure Lengend Snippet: Whole-genome pairwise comparison of SIY1lw and its close-related Salmonella phages 100268_sal2 and OSY-STA using pyGenomeViz. The whole genome map is illustrated as blue arrows with the order of annotated ORFs from left to right along the phage genome. Regions of sequence similarity are connected by a red-scale shaded area. Eleven ORFs with green highlights from SIY1lw are absent on either phage 100268_sal2 or OSY-STA genome. The sequence direction of phage 100268_sal2 is adjusted before the analysis. ORF 43, ORF 44, and ORF 131 encode tail fiber, tail fiber, and ABC transporter permease, respectively; the other ORFs encode hypothetical proteins.

Article Snippet: DNA library preparation was prepared based on the previously published protocol and subsequently loaded on a MiSeq Reagent Kit v2 (500-cycle) for whole-genome sequencing using the MiSeq platform (Illumina, San Deigo, CA, USA).

Techniques: Comparison, Sequencing